Anti-WRNIP1/WHIP antibody

• 产品名称

  Anti-WRNIP1/WHIP抗体
  参阅全部 WRNIP1/WHIP 一抗

• 描述

  兔多克隆抗体to WRNIP1/WHIP

• 宿主

  Rabbit

• 特异性

  Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 浓度

• 批次浓度范围 100 µg 浓度为 1 - 1.1 mg/ml

• 100 µg 浓度为 1.1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase.

• 克隆

  多克隆

• 同种型

  IgG

• The Abpromise guarantee

• Abpromise™承诺保证使用ab4731于以下的经测试应用

• “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 应用	Ab评论	说明
  WB	(1)

• 应用说明

• ELISA: 1/5000 - 1/20000.
  ICC/IF: Use at an assay dependent dilution (PMID 17888034).
  WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).
  
  
  Not yet tested in other applications.
  Optimal dilutions/concentrations should be determined by the end user.

• 数据库链接

• Entrez Gene: 56897 Human

• Entrez Gene: 78903 Mouse

• Entrez Gene: 282835 Rat

• Omim: 608196 Human

• SwissProt: Q96S55 Human

• SwissProt: Q91XU0 Mouse

• SwissProt: Q8CG07 Rat

• Unigene: 236828 Human

• Unigene: 286680 Mouse

• Unigene: 8783 Rat

别名

• ATPase WRNIP 1 antibody

• ATPase WRNIP1 antibody

• bA420G6.2 antibody

• FLJ22526 antibody

• Putative helicase RUVBL antibody

• RP11 420G6.2 antibody

• Werner helicasae interacting protein 1 antibody

• Werner helicasae interacting protein antibody

• Werner helicase interacting protein isoform 2 antibody

• Werner helicase-interacting protein 1 antibody

• WHIP antibody

• WRIP1_HUMAN antibody

• WRNIP 1 antibody

• WRNIP antibody

• Wrnip1 antibody




• 

  Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

  Western blot using ab4731 on HEK 293 lysate at 1/200.

   ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein.  The main band could be blocked using the immunising peptide.

  Western blot using ab4731 on HEK 293 lysate at 1/200.
  
  ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .