Anti-MCM2 antibody

• 产品名称

  Anti-MCM2抗体
  参阅全部 MCM2 一抗

• 描述

  兔多克隆抗体to MCM2

• 宿主

  Rabbit

• 经测试应用

  适用于: IHC-P, IP, WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Chimpanzee, Gorilla, Orangutan

• 免疫原

  Synthetic peptide corresponding to Human MCM2. The epitope recognized maps to a region between residues 1 and 50 of human human minichromosomal maintenance deficient 2 using the numbering given in entry NP_0045117.2 (GeneID 4171).
  Database link: P49736

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 浓度

• 50 µl 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4461于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4171 Human

• Entrez Gene: 17216 Mouse

• Omim: 116945 Human

• SwissProt: P49736 Human

• SwissProt: P97310 Mouse

• Unigene: 477481 Human

• Unigene: 16711 Mouse

• 别名

• CDC like 1 antibody

• cdc19 antibody

• CDCL 1 antibody

• CDCL1 antibody

• Cell devision cycle like 1 antibody

• Cyclin like 1 antibody

• D3S3194 antibody

• DNA replication licensing factor MCM2 antibody

• KIAA0030 antibody

• MCM 2 antibody

• MCM2 antibody

• MCM2 minichromosome maintenance deficient 2 mitotin antibody

• MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody

• MCM2 minichromosome maintenance deficient 2, mitotin antibody

• MCM2_HUMAN antibody

• MGC10606 antibody

• Minichromosome maintenance complex component 2 antibody

• Minichromosome maintenance deficient 2 (mitotin) antibody

• Minichromosome maintenance deficient 2 mitotin antibody

• Minichromosome maintenance protein 2 antibody

• Minichromosome maintenance protein 2 homolog antibody

• Mitotin antibody

• Nuclear protein BM28 antibody

• OTTHUMP00000216047 antibody

• OTTHUMP00000216050 antibody

• BM28 antibody

• CCNL 1 antibody

• CCNL1 antibody

• 

  Western blot - Anti-MCM2 antibody (ab4461)

  All lanes : Anti-MCM2 antibody (ab4461) at 0.1 µg/ml
  
  Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
  Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
  Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
  Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
  Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
  
  Lysates/proteins at 50 µg per lane.
  
  Predicted band size: 100 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody (ab4461)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labeling MCM2 with ab4461 at 1/1000 (1µg/ml). Detection: DAB.

• 

  Immunoprecipitation - Anti-MCM2 antibody (ab4461)

  MCM2 was immunoprecipitated from 1 mg per reaction of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab4461 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab4461 at 1 µg/ml.

  Lane 1: Phospho MCM2 (S108) antibody IP in HEK-293T lysate.
  Lane 2: ab4461 IP in HEK-293T lysate.
  Lane 3: Control IgG instead of ab4461 in HEK-293T lysate.

  
  NETN lysis buffer.
  Exposure time: XXXX.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody (ab4461)

  Ab4461 staining MCM2 in Human placenta.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.