Anti-LAT (phospho Y132)抗体
参阅全部 LAT 一抗
兔多克隆抗体to LAT (phospho Y132)
Rabbit
适用于: WBmore details
与反应: Human
预测可用于: Rat
Synthetic peptide corresponding to Human LAT (phospho Y132).
Jurkat E6.1 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA
浓度
50 µl 浓度为 0.6 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated LAT. The final product is generated by affinity chromatography using an LAT-derived peptide that is phosphorylated at tyrosine 132.
多克隆
IgG
Abpromise™承诺保证使用ab4476于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31.4 kDa). |
Entrez Gene: 27040 Human
Omim: 602354 Human
SwissProt: O43561 Human
Unigene: 632179 Human
Unigene: 9773 Rat
LAT 1 antibody
lat antibody
LAT_HUMAN antibody
LAT1 antibody
Linker for activation of T cells antibody
Linker for activation of T cells family member 1 antibody
linker for activation of T cells, transmembrane adaptor antibody
Linker for activation of T-cells family member 1 antibody
p36 38 antibody
p36-38 antibody
pp36 antibody
36 kDa phospho tyrosine adapter protein antibody
36 kDa phospho-tyrosine adapter protein antibody
36 kDa phospho-tyrosine adaptor protein antibody
Western blot - Anti-LAT (phospho Y132) antibody (ab4476)
Peptide Competition
Extracts prepared from Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5), resolved by SDS-PAGE on a 10% polyacrylamide gel, and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.35 µg/ml phospho LAT (Tyr 132) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to phospho LAT (Tyr 132) completely blocks the antibody signal, thereby demonstrating the specificity of the antibody, and stimulation-induced tyrosine phosphorylation of phospho LAT (Tyr 132).
