Anti-NSP1抗体[32D6]
小鼠单克隆抗体[32D6] to NSP1
Mouse
This antibody was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert bearing ~66% of the C-terminal portion of NSP1. To confirm that 32D6 possessed high affinity for NSP1, strain RS453, which expresses a shortened isoform of NSP1, was compared to the wildtype strain BJ5465. NSP1 in RS453 contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for function and encodes a protein that has been observed to migrate at approxmately 85 kDa on SDS gels. The predicted size of wildtype NSP1 is 86.5 kDa, but NSP1 has been observed to migrate on SDS gels at ~100 kDa. In our gels, the apparent molecular mass of wildtype NSP1 was 108 kDa, whereas the short isoform of NSP1 in RS453 migrated at 91 kDa. The detection of two protein bands of apparent sizes 108 kDa and 91 kDa in BJ5465 and RS453, respectively, demonstrated that 32D6 recognized the pore complex protein NSP1.
适用于: ICC/IF, WBmore details
与反应: Saccharomyces cerevisiae
Tissue, cells or virus corresponding to NSP1. Yeast nuclear preparations
This antibody is a useful marker of yeast nuclear pores.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Tissue culture supernatant
Sterile filtered.
This antibody is a useful marker of yeast nuclear pores.
单克隆
32D6
IgG
Abpromise™承诺保证使用ab4641于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | 1/1000. Yeast cells. |
| WB | 1/10000 - 1/50000. Detects a band of approximately 108,91 kDa (predicted molecular weight: 85 kDa). 1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000. |
NSP1 antibody
Nsp1p antibody
Nuclear pore protein NSP1 antibody
Nucleoporin NSP1 antibody
p110 antibody
![Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)](https://www.abcam.cn/ps/products/4/ab4641/Images/Nsp1p-Primary-antibodies-ab4641-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NSP1 antibody [32D6] (ab4641)Image courtesy of an anonymous Abreview.
ab4641 (1/5000) detecting NSP1 in Saccharomyces cerevisae DDY1810 (green). Cells were fixed in paraformaldehyde, permeabilized in methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue). For further experimental details please refer to Abreview.
抱歉，暂无浏览记录
