Anti-TDP1抗体
参阅全部 TDP1 一抗
兔多克隆抗体to TDP1
Rabbit
适用于: WBmore details
与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.5 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab4166于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa). Abcam recommends blocking with 1-3% Milk. |
Entrez Gene: 55775 Human
Entrez Gene: 104884 Mouse
Omim: 607198 Human
SwissProt: Q9NUW8 Human
SwissProt: Q8BJ37 Mouse
Unigene: 209945 Human
Unigene: 312323 Mouse
Unigene: 487464 Mouse
Unigene: 488658 Mouse
Unigene: 12661 Rat
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Western blot - Anti-TDP1 antibody (ab4166)
Anti-TDP1 antibody (ab4166) at 1 µg/ml (blocked with 3% milk) + Jurkat nuclear extract lysate (ab14844) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDawhy is the actual band size different from the predicted?
Exposure time: 4 minutes
Western blot - Anti-TDP1 antibody (ab4166)This image is courtesy of Keith Caldecott, University of Sussex
Lane 1 : Molecular weight markers
Lanes 2-5 : Anti-TDP1 antibody (ab4166) at 1/1000 dilution
Lane 2 : 1 ng purified recombinant human TDP1
Lane 3 : 3 ng purified recombinant human TDP1
Lane 4 : 6 ng purified recombinant human TDP1
Lane 5 : 6 micrograms total protein from MCF7 human cell extract
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
ab4166 was incubated for 1 hr at room temp.
