Anti-Histone H2A.Z抗体- ChIP Grade
参阅全部 Histone H2A.Z 一抗
兔多克隆抗体to Histone H2A.Z - ChIP Grade
Rabbit
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: ChIP, WBmore details
不适用于: ICC/IF
与反应: Mouse, Rat, Cow, Human
预测可用于: Sheep, Xenopus laevis, Arabidopsis thaliana, Zebrafish
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab11681)
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If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab4174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP | (9) | Use at an assay dependent concentration. |
| WB | (14) | 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 13.4 kDa). Abcam recommends using BSA as the blocking agent. |
应用说明
Is unsuitable for ICC/IF.
Entrez Gene: 3015 Human
Entrez Gene: 51788 Mouse
Omim: 142763 Human
SwissProt: P0C0S5 Human
SwissProt: P0C0S6 Mouse
SwissProt: Q6YNC8 Sheep
Unigene: 119192 Human
Unigene: 117541 Mouse
Unigene: 372513 Mouse
Unigene: 465508 Mouse
Unigene: 112573 Rat
H2A histone family member Z antibody
H2A.z antibody
H2A/z antibody
H2afz antibody
H2AZ antibody
H2AZ_HUMAN antibody
Histone H2A.Z antibody
MGC117173 antibody
ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)Gjidoda et al PLoS One. 2014 Apr 4;9(4):e93971. doi: 10.1371/journal.pone.0093971. eCollection 2014. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Changes in nucleosome occupancy upon LPS induction at a putative distal enhancer and promoter of IL1A, kinetics of nucleosome removal, and changes in histone modifications.
Chromation from mouse bone marrow derived macrophages. ChIP experiments with antibodies against H3 (dark blue bars), H2A.Z (light blue, (ab4174 at 4µg), H3K4me1 (green, ab8895 at 1 µg), H3K4me3 (yellow, ab8580 at 1 µg) and H3K27ac (red, ab4729 at 1 µg). For these experiments cross-linked chromatin was lightly digested with MNase before incubation with the respective antibodies to increase resolution of the ChIP signal and the data was normalized to a region in the ORF of RPL4. Changes upon LPS induction in histone binding and histone modifications at the enhancers and promoters of IL12B and IL1A as well as at a control region in the GAPDH pseudo gene are shown as fold over levels found before induction. For H3K27ac the changes 1.5 h after LPS induction, and for all other histone variants and modifications the changes after 3 h of induction are shown. The error bars show the SEM of at least 3 independent experiments. Statistical significance of the changes in H3K4me3 and H3K27ac upon LPS induction compared to levels found prior to induction determined by Student's T-tests is indicated (*P<0.05; **P<0.01).
ChIP - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)
Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol.
Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4174 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)
Lanes 1-2 : Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/500 dilution
Lanes 3-4 : Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/1000 dilution
Lanes 1 & 3 : Calf thymus histone lysate
Lanes 2 & 4 : Calf thymus histone lysate with Human Histone H2A.Z peptide (ab11681) at 1 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 13.4 kDa
Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)
All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1 µg/ml
Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 4 : PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13.4 kDa
Exposure time: 3 minutes
Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (ab4174)This image is courtesy of an Abreview submitted by Ragnhild Eskeland
All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab4174) at 1/1000 dilution
Lane 1 : Native recombinant octamers K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells at 3 µg
Lane 2 : Native recombinant octamers K562 cells at 1.5 µg
Lane 3 : Recombinant Human octamers containing H2A at 1 µg
Lane 4 : Recombinant Human octamers containing H2A at 0.5 µg
Lane 5 : Recombinant Human octamers containing H2A.Z.2.1 at 0.5 µg
Lane 6 : Recombinant Human octamers containing H2A.Z.1 at 0.5 µg
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13.4 kDa
Exposure time: 5 minutes
Blocked with 3% BSA for 1 hour at 20°C.
Primary incubation in TBS tween + 3% BSA at 20°C for 1 hour.
