Anti-CBX4 antibody

• 产品名称

  Anti-CBX4抗体
  参阅全部 CBX4 一抗

• 描述

  兔多克隆抗体to CBX4

• 宿主

  Rabbit

• 特异性

  In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localisation of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see picture). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody also reacts with another ubiquitous highly expressed protein.

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide:

  GLQDSSTDNRAKLC

  , corresponding to amino acids 95 - 107 of Human PC2.

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 浓度

• 100 µg 浓度为 1.36 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

• Primary antibody说明

  PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4189于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 8535 Human

• Omim: 603079 Human

• SwissProt: O00257 Human

• 别名

• CBX4_HUMAN antibody

• Chromobox homolog 4 (Pc class homolog, Drosophila) antibody

• Chromobox homolog 4 antibody

• Chromobox protein homolog 4 antibody

• E3 SUMO protein ligase CBX 4 antibody

• E3 SUMO protein ligase CBX4 antibody

• E3 SUMO-protein ligase CBX4 antibody

• hPc 2 antibody

• hPc2 antibody

• NBP 16 antibody

• NBP16 antibody

• NS5ATP1 binding protein 16 antibody

• Pc 2 antibody

• Pc class 2 homolog antibody

• Pc class homolog antibody

• Pc class homolog Drosophila antibody

• PC2 antibody

• Polycomb 2 homolog antibody

• CBX 4 antibody

• CBX4 antibody

• Cbx4 chromobox homolog 4 (Drosophila Pc class) antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CBX4 antibody (ab4189)This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

  Immunofluorescent imaging of human cells (U2OS) with ab4189 reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localisation of PC2. This corresponds to results seen in Satijn et al.

  IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C. Nuclei counterstained with Hoechst stain (blue).

• 

  Western blot - Anti-CBX4 antibody (ab4189)This image is courtesy of Dr Ari Melnick, Albert Einstein College of Medicine

  All lanes : Anti-CBX4 antibody (ab4189)
  
  Lane 1 : HEK 293 lysate over-expressing DDDDK tagged hPC2
  Lane 2 : HEK 293 lysate
  
  Predicted band size: 61 kDa
  Observed band size: 75 kDawhy is the actual band size different from the predicted?
  Additional bands at: 30 kDa (possible non-specific secondary antibody binding), 49 kDa (possible non-specific secondary antibody binding), 65 kDa (possible non-specific secondary antibody binding)
  
  
  
  ab4189 specifically recognises over expressed DDDDK tagged hPC2 in HEK293 cells (lane1). The lower bands are assigned to degradation products.