Anti-NUMB抗体
参阅全部 NUMB 一抗
山羊多克隆抗体to NUMB
Goat
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
适用于: ELISA, Flow Cyt, IHC-P, IP, WB, ICC/IFmore details
与反应: Mouse, Chicken, Human
预测可用于: Cow, Dog
Synthetic peptide corresponding to Human NUMB aa 638-651 (C terminal).
Sequence:
C-PFSSDLQKTFEIEL
WB: Mouse brain lysate; ICC: A431 and NIH3T3 cells; Flow Cyt: A431 cells IHC: Human prostate tissue
This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab4147于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 10 µg/ml. | |
| IHC-P | (3) | Use a concentration of 8 µg/ml. |
| IP | (1) | Use at an assay dependent concentration. For 1 hr on mouse brain lysate (see Abreview). |
| WB | (3) | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
| ICC/IF | (3) | Use a concentration of 10 µg/ml. |
Entrez Gene: 8650 Human
Entrez Gene: 18222 Mouse
Omim: 603728 Human
SwissProt: P49757 Human
SwissProt: Q9QZS3 Mouse
Unigene: 654609 Human
Unigene: 714879 Human
Unigene: 4390 Mouse
c14_5527 antibody
C14orf41 antibody
FLJ31314 antibody
h Numb antibody
h-Numb antibody
Numb antibody
Numb homolog (Drosophila) antibody
Numb homolog antibody
Numb protein homolog antibody
NUMB_HUMAN antibody
Protein numb homolog antibody
Protein S171 antibody
S171 antibody
Western blot - Anti-NUMB antibody (ab4147)
Anti-NUMB antibody (ab4147) at 1 µg/ml + Mouse brain lysates at 35 µg
Predicted band size: 71 kDa
Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (ab4147)
Immunohistochemistry staining of paraffin embedded Human Prostate labeling NUMB with ab4147 at (8µg/mL). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry - Anti-NUMB antibody (ab4147)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)This image is courtesy of an anonymous Abreview
ab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
