Anti-Cannabinoid Receptor II抗体
参阅全部 Cannabinoid Receptor II 一抗
兔多克隆抗体to Cannabinoid Receptor II
Rabbit
We have had mixed results for use of this antibody in mouse. Thus, we are removing mouse as a guaranteed application and welcome any feedback from customers who have used this antibody in mouse.
适用于: ICC, WB, IHC-Pmore details
与反应: Rat, Human, Recombinant fragment
Recombinant fragment within Rat Cannabinoid Receptor II aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9QZN9
WB: HT29, C6, rat colon cell lysate; IHC: human tonsil tissue, skin tissue; ICC: AtT20 cells transfected with rat CB2 gene
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
多克隆
IgG
Abpromise™承诺保证使用ab3561于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/500. | |
| WB | (4) | 1/50 - 1/500. Predicted molecular weight: 40 kDa. |
| IHC-P | (2) | 1/10 - 1/100. |
Entrez Gene: 1269 Human
Omim: 605051 Human
SwissProt: P34972 Human
Unigene: 73037 Human
Unigene: 48776 Rat
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hCB2 antibody
testis-dominant CNR2 isoform CB2 antibody
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Cannabinoid receptor 2 macrophage antibody
CB 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution
Lane 1 : HT29 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Rat colon cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)
Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.
