Anti-GNAI1 + GNAI2抗体
兔多克隆抗体to GNAI1 + GNAI2
Rabbit
Similarity with other sequences from the G protein family is predicted but has not being tested empirically.
适用于: IHC-P, ICCmore details
与反应: Human
预测可用于: Chicken, Guinea pig, Hamster, Cow, Dog, Xenopus laevis, Starfish
Synthetic peptide corresponding to Human GNAI1 + GNAI2 aa 300-400.
Database link: P63096
IHC-P: Human tonsil tissue. ICC: SH-SY5Y cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.
多克隆
IgG
Abpromise™承诺保证使用ab3522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | Use a concentration of 2 µg/ml. |
Entrez Gene: 2770 Human
Entrez Gene: 2771 Human
Entrez Gene: 380034 Xenopus laevis
Entrez Gene: 399463 Xenopus laevis
Omim: 139310 Human
Omim: 139360 Human
SwissProt: 38401 Guinea pig
SwissProt: P38402 Guinea pig
SwissProt: P04899 Human
SwissProt: P63096 Human
SwissProt: P27044 Xenopus laevis
Unigene: 134587 Human
Unigene: 1188 Xenopus laevis
Unigene: 21937 Xenopus laevis
Adenylate cyclase-inhibiting G alpha protein antibody
Gi antibody
Gi1 protein alpha subunit antibody
Gi2 protein alpha subunit antibody
GIP antibody
GNAI1 antibody
GNAI1_HUMAN antibody
GNAI2 antibody
GNAI2B antibody
Guanine nucleotide binding protein G(i) alpha 1 subunit antibody
Guanine nucleotide binding protein G(i) alpha 2 subunit antibody
Guanine nucleotide-binding protein G(i) subunit alpha-1 antibody
Immunocytochemistry - Anti-GNAI1 + GNAI2 antibody (ab3522)
SH-SY5Y cells stained for GNAI1 (green) using ab3522 at 2 µg/mL in immunocytochemical analysis. The nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI, F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel e shows the no primary antibody control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI1 + GNAI2 antibody (ab3522)
IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
