Anti-GIPC1抗体
山羊多克隆抗体to GIPC1
Goat
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide corresponding to Human GIPC1 aa 300 to the C-terminus (C terminal). (NP_974197.1; NP_974199.1; NP_974196.1; NP_974198.1; NP_974223.1)
Database link: O14908
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab5951于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use at an assay dependent concentration. |
| WB | (2) | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 10755 Human
Entrez Gene: 67903 Mouse
Omim: 605072 Human
SwissProt: O14908 Human
SwissProt: Q9Z0G0 Mouse
Unigene: 655012 Human
Unigene: 741240 Human
Unigene: 20945 Mouse
Unigene: 30044 Rat
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Western blot - Anti-GIPC1 antibody (ab5951)
ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Western blot - Anti-GIPC1 antibody (ab5951)
Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size: 38 kDa
Observed band size: 24,38 kDawhy is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)This image is courtesy of an anonymous Abreview
ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.
