Anti-ATP1A3 antibody [XVIF9-G10]

• 产品名称

  Anti-ATP1A3抗体[XVIF9-G10]
  参阅全部 ATP1A3 一抗

• 描述

  小鼠单克隆抗体[XVIF9-G10] to ATP1A3

• 宿主

  Mouse

• 特异性

  The immunogen used for this product shares 89% homology with ATP1A2. Cross-reactivity with this protein has not been confirmed experimentally

• 经测试应用

  适用于: WB, Flow Cyt, ICC/IF, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Non human primates, Amphibian, Shark

• 免疫原

  Full length protein corresponding to Dog ATP1A3. Canine cardiac microsomes.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.05% Sodium azide
  Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  XVIF9-G10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2826于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 478 Human

• Entrez Gene: 232975 Mouse

• Entrez Gene: 24213 Rat

• Omim: 182350 Human

• SwissProt: P13637 Human

• SwissProt: Q6PIC6 Mouse

• SwissProt: P06687 Rat

• Unigene: 515427 Human

• Unigene: 44101 Mouse

• Unigene: 87329 Rat

别名

• AHC2 antibody

• Alpha(III) antibody

• AT1A3_HUMAN antibody

• Atp1a3 antibody

• ATPase Na+/K+ transporting alpha 3 polypeptide antibody

• DYT 12 antibody

• DYT12 antibody

• MGC13276 antibody

• Na(+)/K(+) ATPase alpha(III) subunit antibody

• Na(+)/K(+) ATPase alpha-3 subunit antibody

• Na+/K+ ATPase 3 antibody

• Na+/K+ ATPase alpha 3 subunit antibody

• RDP antibody

• Sodium potassium ATPase alpha 3 polypeptide antibody

• Sodium pump 3 antibody

• Sodium pump subunit alpha-3 antibody

• Sodium/potassium transporting ATPase alpha 3 chain antibody

• Sodium/potassium-transporting ATPase subunit alpha-3 antibody

• 

  Western blot - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  All lanes : Anti-ATP1A3 antibody [XVIF9-G10] (ab2826) at 1 µg/ml
  
  Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
  Lane 2 : IMR-32 (Human brain neuroblast cell line) whole cell lysate
  Lane 3 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
  Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
  Lane 5 : Mouse brain tissue lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
  
  Predicted band size: 111 kDa
  
  
  
  

  Samples were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a Nitrocellulose membrane by iBlot® 2 Dry Blotting System. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.

• 

  Flow Cytometry - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  Overlay histogram showing SH-SY5Y cells stained with ab2826 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2826, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at 1/50 dilution with ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at 1/200 dilution with ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at 1/200 dilution with ab2826 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using ab2826 shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 °C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using ab2826 shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4 °C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATP1A3 antibody [XVIF9-G10] (ab2826)

  Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using ab2826 shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 ab2826 at a dilution of 1:20 over night at 4°C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.