Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody

• 产品名称

  Anti-PAK1 (phospho T423) + PAK2 (phospho T402) + PAK3 (phospho T436)抗体

• 描述

  兔多克隆抗体to PAK1 (phospho T423) + PAK2 (phospho T402) + PAK3 (phospho T436)

• 宿主

  Rabbit

• 特异性

  This affinity purified antibody is directed against human PAK (p21-GTPase Activated Protein Kinase). This phospho specific polyclonal antibody is specific for phosphorylated pT423 of Human PAK 1/2/3. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA. Cross reactivity with PAK 1/2/3 from other species has not been determined.

• 经测试应用

  适用于: Dot blot, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to Human PAK1 (phospho T402 + T423 + T436) conjugated to keyhole limpet haemocyanin. Corresponding to amino acids 413-433 of Human PAK 1, 408-428 of human PAK 2 and 411-431 of human PAK 3. Conjugated to Keyhole Limpet Hemocyanin (KLH).

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• 常规说明

  
  

  P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 纯度

  Immunogen affinity purified

• 纯化说明

  This affinity purified antibody is directed against human PAK (p21-GTPase Activated Protein Kinase). The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

• Primary antibody说明

  P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2477于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 5058 Human

• Entrez Gene: 5062 Human

• Entrez Gene: 5063 Human

• GenBank: NP_002568.2 Human

• Omim: 300142 Human

• Omim: 602590 Human

• Omim: 605022 Human

• SwissProt: O75914 Human

• SwissProt: Q13153 Human

• SwissProt: Q13177 Human

• Unigene: 435714 Human

• Unigene: 518530 Human

• 

  Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)

  Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.

• 

  Dot Blot - Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)

  Dot Blot analysis labeling phosphorylated and non-phosphorylated forms of the immunizing peptide with ab2477 at 1/1000 dilution, incubated overnight at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1/40000 dilution for 45 min at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT. 

  Load: 200 ng, 66.7 ng, 22.2 ng, 7.4 ng, or 2.5 ng