Anti-CD105 antibody [MEM-226]

• 产品名称

  Anti-CD105抗体[MEM-226]
  参阅全部 CD105 一抗

• 描述

  小鼠单克隆抗体[MEM-226] to CD105

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, Sandwich ELISA, Flow Cyt, WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein (Human). Expressed in vaccinia virus containing CD105 cDNA.

• 阳性对照

• ICC/IF: HeLa cells. WB: Human colon tissue lysate. Flow Cyt: U937 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from TCS. Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  MEM-226

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2529于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2022 Human

• Omim: 131195 Human

• SwissProt: P17813 Human

• Unigene: 76753 Human

• 别名

• CD105 antibody

• CD105 antigen antibody

• EGLN_HUMAN antibody

• END antibody

• Endoglin antibody

• Eng antibody

• FLJ41744 antibody

• HHT1 antibody

• ORW antibody

• ORW1 antibody

• Osler Rendu Weber syndrome 1 antibody

• RP11 228B15.2 antibody

• S endoglin antibody

• S-endoglin antibody

• SN6 antibody

• AI528660 antibody

• AI662476 antibody

• CD 105 antibody

• 

  Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)

  Flow cytometry analysis showing separation of HUVEC cells stained using ab2529 (concentration in sample 1.67 μg/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed).

• 

  Western blot - Anti-CD105 antibody [MEM-226] (ab2529)

  All lanes : Anti-CD105 antibody [MEM-226] (ab2529) at 1/1000 dilution
  
  Lane 1 : Wild-type HeLa whole cell lysate
  Lane 2 : CD105 knockout HeLa whole cell lysate
  Lane 3 : HUVEC whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  
  

  Lanes 1 - 3: Merged signal (red and green). Green - ab2529 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab2529 was shown to recognize ENG (Endoglin) in wild-type HeLa cells as signal was lost at the expected MW in ENG knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ENG knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab2529 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)

  Overlay histogram showing U937 (Human histiocytic lymphoma cell line) cells stained with ab2529 (red line).

  The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2529, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)

  ICC/IF image of ab2529 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2529, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was ab69879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

• 

  Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)

  Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.

• 

  Western blot - Anti-CD105 antibody [MEM-226] (ab2529)

  Anti-CD105 antibody [MEM-226] (ab2529) at 5 µg/ml + Human colon tissue lysate at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Observed band size: 80 kDawhy is the actual band size different from the predicted?
  Additional bands at: 45 kDa, 55 kDa. We are unsure as to the identity of these extra bands.