Anti-Prolactin Receptor/PRL-R antibody [T6]

• 产品名称

  Anti-Prolactin Receptor/PRL-R抗体[T6]
  参阅全部 Prolactin Receptor/PRL-R 一抗

• 描述

  小鼠单克隆抗体[T6] to Prolactin Receptor/PRL-R

• 宿主

  Mouse

• 特异性

  Detects Prolactin Receptor/PRL-R in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing Prolactin Receptor/PRL-R in NB2 cell lysate. Immunohistochemical staining of Prolactin Receptor/PRL-R in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to Prolactin Receptor/PRL-R in vitro.

• 经测试应用

  适用于: IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Rat, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Rat Prolactin Receptor/PRL-R. Purified rat liver PRL receptor.

• 阳性对照

• IHC-P: Rat pituitary gland tissue. ICC: C6, H-4-II-E, SW480 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 克隆

  单克隆

• 克隆编号

  T6

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab2773于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5618 Human

• Entrez Gene: 24684 Rat

• Omim: 176761 Human

• SwissProt: P16471 Human

• SwissProt: P05710 Rat

• Unigene: 368587 Human

• Unigene: 9757 Rat

• 别名

• Delta 4-delta 7/11 truncated prolactin receptor antibody

• Delta 4-SF1b truncated prolactin receptor antibody

• HPRL antibody

• hPRL receptor antibody

• hPRLrI antibody

• Lactogen receptor antibody

• MFAB antibody

• MGC105486 antibody

• OPR antibody

• OTTHUMP00000115998 antibody

• Pr-1 antibody

• Pr-3 antibody

• PRL R antibody

• PRL-R antibody

• PRLR antibody

• Prlr-rs1 antibody

• PRLR_HUMAN antibody

• Prolactin receptor a antibody

• Prolactin receptor antibody

• Prolactin receptor delta 7/11 antibody

• RATPRLR antibody

• Secreted prolactin binding protein antibody

• Truncated testis-specific box 1-C prolactin receptor antibody

• wu:fj65c07 antibody

• AI987712 antibody

• CLONE SPM213 antibody

• CPRLP antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

  Immunocytochemical analysis of C6 (Rat glial tumor cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

  Immunocytochemical analysis of H-4-II-E (Rat hepatoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

  Immunocytochemical analysis of in SW480 (Human colorectal adenocarcinoma cell line) cells labeling Prolactin Receptor/PRL-R. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor/PRL-R staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [T6] (ab2773)

  Immunohistochemical analysis of normal biopsies of deparaffinized rat pituitary gland tissue labeling Prolactin Receptor/PRL-R. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.