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Anti-SRC3 antibody [AX15.3] - ChIP Grade

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  • 产品名称

    Anti-SRC3抗体[AX15.3] - ChIP Grade
    参阅全部 SRC3 一抗

  • 描述

    小鼠单克隆抗体[AX15.3] to SRC3 - ChIP Grade

  • 宿主

    Mouse

  • 经测试应用

    适用于: WBIHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment (GST-tag) corresponding to Human SRC3 aa 600-1300.

  • 阳性对照

    • WB: MCF-7 cell lysate, IHC-P: Human pancreas tissue.

  • 常规说明

    Locus Link: 8202. Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1. Alias: Amplified in breast cancer-1.


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS

  • 纯度

    Affinity purified

  • 克隆

    单克隆

  • 克隆编号

    AX15.3

  • 同种型

    IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2782于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
WB(1)

Use a concentration of 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).

IHC-P

1/10 - 1/100.

  • 数据库链接

  • 别名

    • AIB1 antibody

    • Amplified in breast cancer 1 antibody

    • Amplified in breast cancer 1 protein antibody

    • bHLHe42 antibody

    • CAGH 16 antibody

    • CAGH16 antibody

    • CBP interacting protein antibody

    • CBP-interacting protein antibody

    • Class E basic helix-loop-helix protein 42 antibody

    • CTG 26 antibody

    • CTG26 antibody

    • KAT13B / AIB1 antibody

    • MGC141848 antibody

    • NCOA 3 antibody

    • NCoA-3 antibody

    • Ncoa3 antibody

    • NCOA3_HUMAN antibody

    • Nuclear receptor coactivator 3 antibody

    • pCIP antibody

    • RAC 3 antibody

    • RAC-3 antibody

    • RAC3 antibody

    • Receptor associated coactivator 3 antibody

    • Receptor-associated coactivator 3 antibody

    • SRC 3 antibody

    • SRC-3 antibody

    • SRC3 antibody

    • Steroid receptor coactivator protein 3 antibody

    • Thyroid hormone receptor activator molecule 1 antibody

    • TNRC14 antibody

    • TNRC16 antibody

    • TRAM 1 antibody

    • TRAM-1 antibody

    • TRAM1 antibody

    • ACTR antibody

    • AIB 1 antibody

    • AIB-1 antibody

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human pancreas tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2782 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting

  • Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

    Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

    Western blot of AIB1 on MCF-7 cell extract using ab2782. Western blot of AIB1 on MCF-7 cell extract using ab2782.