Anti-Y14 antibody [4C4]

• 产品名称

  Anti-Y14抗体[4C4]
  参阅全部 Y14 一抗

• 描述

  小鼠单克隆抗体[4C4] to Y14

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, WB, ICC, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified) (Human).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

  
  

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• 纯度

  Protein A purified

• 纯化说明

  Purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  4C4

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2b

• 研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab5828于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 9939 Human

• Omim: 605313 Human

• SwissProt: Q9Y5S9 Human

• Unigene: 728824 Human

• 别名

• BOV 1A antibody

• BOV 1B antibody

• BOV 1C antibody

• BOV-1 antibody

• BOV1 antibody

• BOV1A antibody

• BOV1B antibody

• BOV1C antibody

• HSPC 114 antibody

• HSPC114 antibody

• MDS 014 antibody

• MDS014 antibody

• RBM 8 antibody

• RBM 8A antibody

• RBM 8B antibody

• RBM8 antibody

• rbm8a antibody

• RBM8A_HUMAN antibody

• RBM8B antibody

• Ribonucleoprotein RBM 8 antibody

• Ribonucleoprotein RBM 8A antibody

• Ribonucleoprotein RBM8 antibody

• Ribonucleoprotein RBM8A antibody

• RNA binding motif protein 8 antibody

• RNA binding motif protein 8A antibody

• RNA binding motif protein 8B antibody

• RNA binding protein 8A antibody

• RNA binding protein Y14 antibody

• RNA-binding motif protein 8A antibody

• RNA-binding protein 8A antibody

• RNA-binding protein Y14 antibody

• ZNRP antibody

• ZRNP 1 antibody

• ZRNP1 antibody

• Binder of OVCA1 1 antibody

• Binder of OVCA1-1 antibody

• BOV 1 antibody

• 

  Western blot - Anti-Y14 antibody [4C4] (ab5828)

  All lanes : Anti-Y14 antibody [4C4] (ab5828) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 19 kDa
  Observed band size: 21 kDawhy is the actual band size different from the predicted?
  Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 8 minutes
  

• 

  Immunocytochemistry - Anti-Y14 antibody [4C4] (ab5828)

  ICC/IF image of ab5828 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5828, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Y14 antibody [4C4] (ab5828)

  IHC image of ab5828 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Flow Cytometry - Anti-Y14 antibody [4C4] (ab5828)

  Overlay histogram showing HeLa cells stained with ab5828 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5828, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.