Anti-beta Catenin antibody

• 产品名称

  Anti-beta Catenin抗体
  参阅全部 beta Catenin 一抗

• 描述

  兔多克隆抗体to beta Catenin

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, IHC-P, IPmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Chicken, Xenopus laevis

• 免疫原

  Synthetic peptide within Human beta Catenin aa 750 to the C-terminus. The exact sequence is proprietary.
  Database link: P35222

• 常规说明

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.60
  Preservative: 0.1% Sodium azide
  Constituents: PBS, 1% BSA

• 浓度

• 批次浓度范围 500 µl 浓度为 0.07 - 0.625 mg/ml

• 1 ml 浓度为 0.07 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2365于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 395964 Chicken

• Entrez Gene: 1499 Human

• Entrez Gene: 12387 Mouse

• Entrez Gene: 84353 Rat

• Omim: 116806 Human

• SwissProt: P35222 Human

• SwissProt: Q02248 Mouse

• SwissProt: Q9WU82 Rat

• SwissProt: P26233 Xenopus laevis

• Unigene: 476018 Human

• Unigene: 291928 Mouse

• Unigene: 112601 Rat

别名

• b-catenin antibody

• Beta catenin antibody

• Beta-catenin antibody

• Cadherin associated protein antibody

• Catenin (cadherin associated protein), beta 1, 88kDa antibody

• Catenin beta 1 antibody

• Catenin beta-1 antibody

• CATNB antibody

• CHBCAT antibody

• CTNB1_HUMAN antibody

• CTNNB antibody

• CTNNB1 antibody

• DKFZp686D02253 antibody

• FLJ25606 antibody

• FLJ37923 antibody

• OTTHUMP00000162082 antibody

• OTTHUMP00000165222 antibody

• OTTHUMP00000165223 antibody

• OTTHUMP00000209288 antibody

• OTTHUMP00000209289 antibody

• 

  Western blot - Anti-beta Catenin antibody (ab2365)

  Anti-beta Catenin antibody (ab2365) at 1/25 dilution + A431 cell lysate
  
  Predicted band size: 85 kDa
  Observed band size: 92 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab2365)

  Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for beta Catenin using ab2365 at 1/250 dilution in immunohistochemical analysis.

• 

  Immunoprecipitation - Anti-beta Catenin antibody (ab2365)

  Beta Catenin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to beta Catenin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2365.
  Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
  Band: 85kDa; beta Catenin.