Anti-VEGF Receptor 1抗体
参阅全部 VEGF Receptor 1 一抗
兔多克隆抗体to VEGF Receptor 1
Rabbit
适用于: IHC-P, ICC/IFmore details
与反应: Human
预测可用于: Pig
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
IHC-P: Human angiosarcoma tissue. ICC/IF: A431 cell line
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
浓度
批次浓度范围 100 µl 浓度为 0.4 - 0.42 mg/ml
500 µl 浓度为 0.42 mg/ml
Immunogen affinity purified
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
多克隆
IgG
Abpromise™承诺保证使用ab2350于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) | 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| ICC/IF | Use a concentration of 5 µg/ml. |
Entrez Gene: 2321 Human
Omim: 165070 Human
SwissProt: P17948 Human
Unigene: 594454 Human
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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)
ab2350 - immunohistochemistry
Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.
