FITC Anti-CD90 / Thy1 antibody [MRC OX-7]

• 产品名称

  FITC荧光Anti-CD90 / Thy1抗体[MRC OX-7]
  参阅全部 CD90 / Thy1 一抗

• 描述

  FITC荧光小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1

• 宿主

  Mouse

• 偶联物

  FITC. Ex: 493nm, Em: 528nm

• 特异性

  Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c.

• 经测试应用

  适用于: Flow Cyt (Intra), ICCmore details

• 种属反应性

  与反应: Rat
  预测可用于: Mouse, Rabbit, Guinea pig

• 免疫原

  Full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• ICC: PC12 cells. Flow Cyt (Intra): PC12 cells.

• 常规说明

  The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

  Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

• 浓度

• 50 µg 浓度为 0.5 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  MRC OX-7

• 骨髓瘤

  NS1

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab226于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 21838 Mouse

• Entrez Gene: 24832 Rat

• SwissProt: P01831 Mouse

• SwissProt: P01830 Rat

• Unigene: 3951 Mouse

• Unigene: 108198 Rat

• 别名

• CDw90 antibody

• FLJ33325 antibody

• MGC128895 antibody

• T25 antibody

• Theta antigen antibody

• Thy 1 antibody

• Thy 1 cell surface antigen antibody

• Thy 1 membrane glycoprotein antibody

• Thy 1 T cell antigen antibody

• Thy 1.2 antibody

• Thy-1 antigen antibody

• Thy-1 membrane glycoprotein antibody

• Thy1 antibody

• Thy1 antigen antibody

• Thy1 T cell antigen antibody

• Thy1.1 antibody

• Thy1.2 antibody

• THY1_HUMAN antibody

• Thymus cell antigen 1, theta antibody

• CD7 antibody

• CD90 antibody

• CD90 antigen antibody

图片

• 

  Immunocytochemistry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  ab226 staining CD90 / Thy1 in undifferentiated PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI(shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

• 

  Immunocytochemistry - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min at 22°C.

  Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  This antibody gave a positive signal in PC12 (undifferentiated) cells fixed with 4 &#37 formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

• 

  Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min on ice.

  Isotype control antibody (black line) was  used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

• 

  Flow Cytometry (Intracellular) - FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)

  Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.

  Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.