Anti-BRG1抗体
参阅全部 BRG1 一抗
兔多克隆抗体to BRG1
Rabbit
We have conflicting data about the performance of this antibody in ChIP. Publications have reported positive results with this antibody using ChIP application, however we also have customer data indicating certain batches of this antibody did not work in ChIP in their hands. If you require any further information or assistance please contact Abcam Scientific Support Team.
适用于: WBmore details
不适用于: IHC-P
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.7 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab4081于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | 1/500 - 1/1000. Detects a band of approximately 235 kDa (predicted molecular weight: 185 kDa). |
应用说明
Is unsuitable for IHC-P.
Entrez Gene: 6597 Human
Entrez Gene: 20586 Mouse
Omim: 603254 Human
SwissProt: P51532 Human
SwissProt: Q3TKT4 Mouse
Unigene: 327527 Human
Unigene: 286593 Mouse
Unigene: 23417 Rat
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SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody
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Western blot - Anti-BRG1 antibody (ab4081)
All lanes : Anti-BRG1 antibody (ab4081) at 1/500 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : BRG1 knockout HAP1 cell lysate
Lane 3 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 185 kDa
Lanes 1 - 4: Merged signal (red and green).
Green - ab4081 observed at 240 kDa.
Red - loading control, Mouse monoclonal Anti-Vinculin antibody, observed at 124 kDa.
ab4081 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands.
Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. Ab4081 and loading control to Vinculin were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773 - Green) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776 - Red) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-BRG1 antibody (ab4081)
All lanes : Anti-BRG1 antibody (ab4081) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat nuclear extract lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Hep G2 nuclear extract lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 235 kDawhy is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-BRG1 antibody (ab4081)
All lanes : Anti-BRG1 antibody (ab4081) at 1/500 dilution
Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) Nuclear extract
Lane 2 : HeLa Nuclear extract with Human BRG1 peptide (ab13736) at 1 µg
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) (Goat anti-rabbit (HRP))
Predicted band size: 185 kDa
Western blot - Anti-BRG1 antibody (ab4081)
Anti-BRG1 antibody (ab4081) at 1/1000 dilution + Recombinant human BRG1 protein (ab82237) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 185 kDa
Exposure time: 8 minutes
Western blot - Anti-BRG1 antibody (ab4081)This image is courtesy of an anonymous abreview.
Whole cell lysate prepared from HeLa cells was loaded at 150000 cells.
ab4081 used at a 1/1000 dilution.
The secondary used was an HRP conjugated donkey polyclonal used at a 1/5000 dilution.
