Anti-RACGAP1/MGCRACGAP抗体
参阅全部 RACGAP1/MGCRACGAP 一抗
山羊多克隆抗体to RACGAP1/MGCRACGAP
Goat
适用于: IHC-P, ICC, WBmore details
与反应: Human
预测可用于: Cow, Dog
Synthetic peptide corresponding to Human RACGAP1/MGCRACGAP aa 600 to the C-terminus (C terminal). (NP_001306934.1; NP_001306935.1; NP_001306936.1)
Database link: Q9H0H5
WB: Human testis tissue lysate. K562 and Jurkat cell lysates. ICC: MCF7 cells. IHC-P: Human testis tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab2270于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. | |
| ICC | Use a concentration of 10 µg/ml. | |
| WB | (2) | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 29127 Human
Omim: 604980 Human
SwissProt: Q9H0H5 Human
Unigene: 505469 Human
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Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)
All lanes :
Lane 1 : K562 cell lysate (in RIPA buffer)
Lane 2 : Jurkat cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 71 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab2270)
Paraffin embedded human testis tissue stained for RACGAP1/MGCRACGAP using ab2270 at 4 µg/ml in immunohistochemical analysis.
Immunocytochemistry - Anti-RACGAP1/MGCRACGAP antibody (ab2270)
Immunoctochemistry analysis of paraformaldehyde fixed MCF7 (human breast adenocarcinoma cell line) cells labeling RACGAP1/MGCRACGAP with ab2270 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour followed by Alexa Fluor® 488 secondary antibody (4 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (4 µg/mL).
Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)
Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 0.2 µg/ml + Human Testis lysate (RIPA buffer) at 30 µg
Predicted band size: 71 kDa
ab2270 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)
Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 1 µg/ml + K562 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Observed band size: 75 kDa
