Anti-Histone H1.2抗体
参阅全部 Histone H1.2 一抗
兔多克隆抗体to Histone H1.2
Rabbit
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: ICC/IF, IP, WBmore details
与反应: Mouse, Human, Recombinant fragment
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
This antibody gave a positive signal in Human H1.2 Recombinant Protein. ICC/IF: HeLa cell line
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17677于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) | Use a concentration of 1 µg/ml. |
| IP | Use a concentration of 5 µg/ml. | |
| WB | (2) | 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Entrez Gene: 3006 Human
Entrez Gene: 50708 Mouse
Omim: 142710 Human
SwissProt: P16403 Human
SwissProt: P15864 Mouse
Unigene: 7644 Human
Unigene: 193539 Mouse
H1F2 antibody
H1s-1 antibody
HIST1H1C antibody
Histone 1 H1c antibody
Histone cluster 1 H1c antibody
Histone H1.2 antibody
Histone H1c antibody
Histone H1d antibody
Histone H1s-1 antibody
MGC3992 antibody
H1 histone family member 2 antibody
H1.a antibody
H12_HUMAN antibody
Western blot - Anti-Histone H1.2 antibody (ab17677)
All lanes : Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HIST1H1C knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 32 kDawhy is the actual band size different from the predicted?
False colour image of Western blot: Anti-Histone H1.2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab17677 was shown to bind specifically to Histone H1.2. A band was observed at 32 kDa in wild-type HeLa cell lysates with no signal observed at this size in HIST1H1C knockout cell line ab261794 (knockout cell lysate ab257218). To generate this image, wild-type and HIST1H1C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-Histone H1.2 antibody (ab17677)This image is courtesy of Dr Albert Jordan and Monica Sancho, Center for Genomic Regulation (CRG). Recombinant H1 isoforms courtesy of Dr Nicole Happel.
All lanes : Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilution
Lane 1 : Recombinant histone H1
Lane 2 : Recombinant histone H1.1
Lane 3 : Recombinant histone H1.2
Lane 4 : Recombinant histone H1.3
Lane 5 : Recombinant histone H1.4
Lane 6 : Recombinant histone H1.5
Predicted band size: 29 kDa
Observed band size: 29 kDa
Immunoprecipitation - Anti-Histone H1.2 antibody (ab17677)
Histone H1.2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H1.2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17677.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 35kDa; Histone H1.2
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.2 antibody (ab17677)
ab17677 staining Histone H1.2 in HeLa cells. The cells were fixed with 100% methanol (5 min) at room temperature, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tritonn for 1h. The cells were then incubated with the antibody ab16766 at 0.1µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
Western blot - Anti-Histone H1.2 antibody (ab17677)
All lanes : Anti-Histone H1.2 antibody (ab17677) at 1 µg/ml
Lane 1 : Histone H1 Recombinant Protein
Lane 2 : Histone H1.2 (Human) - Recombinant Protein
Lane 3 : Histone H1 Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/ml
Lane 4 : Histone H1.2 (Human) - Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/ml
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 12 minutes
