Anti-Nestin antibody [2C1.3A11]

• 产品名称

  Anti-Nestin抗体[2C1.3A11]
  参阅全部 Nestin 一抗

• 描述

  小鼠单克隆抗体[2C1.3A11] to Nestin

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WB, Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Human
  不与反应: Rat

• 免疫原

  Recombinant fragment corresponding to Human Nestin aa 1450-1650.

• 常规说明

  
  

  This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein G purified

• Primary antibody说明

  This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

• 克隆

  单克隆

• 克隆编号

  2C1.3A11

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab18102于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 10763 Human

• Omim: 600915 Human

• SwissProt: P48681 Human

• Unigene: 527971 Human

• 别名

• Intermediate filament protein antibody

• Nbla00170 antibody

• nes antibody

• NEST_HUMAN antibody

• Nestin antibody

• ESTM 46 antibody

• FLJ 21841 antibody

• FLJ21841 antibody

• 

  Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)

  All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/5000 dilution
  
  Lane 1 : Wild-type HAP1 whole cell lysate
  Lane 2 : NES knockout HAP1 whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Predicted band size: 177 kDa
  
  
  
  

  Lanes 1 - 2: Merged signal (red and green). Green - ab18102 observed at 300 kDa. Red - loading control, ab176560, observed at 50 kDa.

  ab18102 was shown to recognize NES in wild-type HAP1 cells as signal was lost at the expected MW in NES knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. Ab18102 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)

  Immunofluorescence staining of U251 cells (glioblastoma cell line) stained using ab18102(green) and bisbenzimide (blue)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of migrating tumor cells labelling Nestin with ab18102 at 2µg/ml. Cytoplasm and membrane positive staining shown.

• 

  Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)

  All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/1000 dilution
  
  Lane 1 : Total rat brain protein at 5 µg
  Lane 2 : Human CNS progenitor cell protein at 1 µg
  Lane 3 : Human CNS progenitor cell protein at 5 µg
  
  Predicted band size: 177 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of xenograft tumor cells labelling Nestin with ab18102.

• 

  Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)

  Overlay histogram showing SH-SY5Y cells stained with ab18102 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18102, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

  Immunohistochemistry of Anti-Nestin on formalin fixed paraffin embedded prostate nerve tissue using ab18102