Anti-KDM1/LSD1抗体-核Marker
参阅全部 KDM1/LSD1 一抗
兔多克隆抗体to KDM1/LSD1 -核Marker
Rabbit
Replenishment batches of our polyclonal antibody, ab17721 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab129195.
适用于: ICC/IF, WBmore details
与反应: Mouse, Rat, Human
预测可用于: Pig
Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human LSD1.
参阅Abcam的专有抗源政策 (Peptide available as ab17763.)
ICC/IF: HeLa, 293T, c2c12 cells. WB: Jurkat, HCT 116, NIH/3T3, C2C12, Mouse skeletal muscle, C6, HeLa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17721于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (4) | Use a concentration of 1 µg/ml. |
| WB | (12) | Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa). |
Entrez Gene: 23028 Human
Entrez Gene: 99982 Mouse
Omim: 609132 Human
SwissProt: O60341 Human
SwissProt: Q6ZQ88 Mouse
Unigene: 591518 Human
Unigene: 28540 Mouse
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Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: KDM1 / LSD1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab17721 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab17721 was shown to specifically react with KDMI/LSD1 in wild-type HAP1 cells. No band was observed when KDMI/LSD1 knockout samples were examined.
Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
ab17721 staining KDM1 / LSD1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17721 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721)
All lanes :
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
Lane 2 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5 : Mouse skeletal muscle lysates at 20 µg
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 110 kDawhy is the actual band size different from the predicted?
Exposure time: 50 seconds
Blocking Buffer & Diluting buffer concentration: 5% NFDM/TBST
Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)
All lanes : Anti-KDM1/LSD1 antibody - Nuclear Marker (ab17721) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat whole cell lysate (ab7899)
Lane 3 : NIH/3T3 whole cell lysate (ab7179)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 93 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)Image courtesy of anonymous abreviewer
ab17721 staining LSD1 in the mouse c2c12 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A diluted (1/2000) Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-KDM1/LSD1 antibody - Nuclear Marker and ChIP Grade (ab17721)This image is courtesy of Katia Ancelin
Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.
