Anti-Caveolin-1抗体- Caveolae Marker
参阅全部 Caveolin-1 一抗
兔多克隆抗体to Caveolin-1 - Caveolae Marker
Rabbit
适用于: IP, WBmore details
不适用于: ICC/IF
与反应: Mouse, Rat, Human, Recombinant fragment
预测可用于: Cow, Dog, Pig
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.3 - 0.9 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab18199于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | (5) | Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
应用说明
Is unsuitable for ICC/IF.
hide
SwissProt: Q03135 Human
SwissProt: P49817 Mouse
Unigene: 74034 Human
Unigene: 28278 Mouse
Unigene: 470537 Mouse
Unigene: 22518 Rat
Entrez Gene: 857 Human
Entrez Gene: 12389 Mouse
Omim: 601047 Human
CAV1_HUMAN antibody
caveolae protein, 22 kD antibody
caveolin 1 alpha isoform antibody
caveolin 1 beta isoform antibody
Caveolin 1 caveolae protein 22kDa antibody
Caveolin-1 antibody
Caveolin1 antibody
cell growth-inhibiting protein 32 antibody
CGL3 antibody
LCCNS antibody
MSTP085 antibody
OTTHUMP00000025031 antibody
PPH3 antibody
VIP 21 antibody
VIP21 antibody
BSCL3 antibody
CAV antibody
CAV1 antibody
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 21-24 kDawhy is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green - ab18199 observed at 21-24 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab18199 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab18199 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : Mouse Heart Lysate
Lane 2 : Human Heart Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
Caveolin 1 - Caveolae Marker was immunoprecipitated using 0.5mg Mouse Heart whole tissue lysate, 5µg of Rabbit polyclonal to Caveolin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18199.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 20kDa: Caveolin 1 - Caveolae Marker.
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml + Recombinant Human Caveolin-1 protein (ab114170) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Exposure time: 1 minute
ab18199 recognizes the full length tagged recombinant Caveolin 1 protein (ab114170) which has an expected molecular weight of 46 kDa.
