Anti-COX2 / Cyclooxygenase 2抗体[SP21]
参阅全部 COX2 / Cyclooxygenase 2 一抗
兔单克隆抗体[SP21] to COX2 / Cyclooxygenase 2
Rabbit
适用于: IHC-Pmore details
不适用于: WB
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 (C terminal). The exact sequence is proprietary.
IHC-P: Human colon carcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
浓度
1 ml 浓度为 0.006 mg/ml
500 µl 浓度为 0.006 mg/ml
100 µl 浓度为 0.006 mg/ml
500 µl 浓度为 0.006 mg/ml
100 µl 浓度为 0.006 mg/ml
40 µl 浓度为 0.006 mg/ml
20 µl 浓度为 0.006 mg/ml
Protein A purified
单克隆
SP21
IgG
Abpromise™承诺保证使用ab16701于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for WB.
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Unigene: 292547 Mouse
Unigene: 44369 Rat
Entrez Gene: 5743 Human
Entrez Gene: 19225 Mouse
Omim: 600262 Human
SwissProt: P35354 Human
SwissProt: Q05769 Mouse
Unigene: 196384 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma labeling COX2/Cyclooxygenase 2 with ab16701 at 1/100 (6 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16701 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.