Anti-hair cortex Cytokeratin/K40 antibody [AE13]

• 产品名称

  Anti-hair cortex Cytokeratin/K40抗体[AE13]
  参阅全部 hair cortex Cytokeratin/K40 一抗

• 描述

  小鼠单克隆抗体[AE13] to hair cortex Cytokeratin/K40

• 宿主

  Mouse

• 特异性

  This antibody is specific for the family of hair cortex keratins.

• 经测试应用

  适用于: IHC-Fr, IHC-P, WB, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human hair cortex Cytokeratin/K40.

• 常规说明

  This antibody is an excellent marker for hair and nail differentiation.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.20
  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein G purified

• Primary antibody说明

  This antibody is an excellent marker for hair and nail differentiation.

• 克隆

  单克隆

• 克隆编号

  AE13

• 骨髓瘤

  P3x63-Ag8.653

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab16113于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 125115 Human

• Entrez Gene: 406221 Mouse

• SwissProt: Q6A162 Human

• SwissProt: Q6IFX3 Mouse

• Unigene: 534929 Human

• Unigene: 422372 Mouse

• 别名

• K1C40_HUMAN antibody

• K40 antibody

• Keratin antibody

• Keratin, type I cytoskeletal 40 antibody

• Keratin-40 antibody

• Krt40 antibody

• type I cytoskeletal 40 antibody

• Type I hair keratin Ka36 antibody

• CK-40 antibody

• Cytokeratin-40 antibody

• hair cortex Cytokeratin antibody

• 

  Western blot - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)

  All lanes : Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113) at 1 µg/ml
  
  Lane 1 : Mouse skin tissue lysate (14 days) (ab7271)
  Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 44 - 46 kDa
  Observed band size: 44 kDawhy is the actual band size different from the predicted?
  Additional bands at: 25 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 20 minutes
  

• 

  Flow Cytometry - Anti-hair cortex Cytokeratin/K40 antibody [AE13] (ab16113)

  Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.