Anti-SSEA4 antibody [MC813-70]

• 产品名称

  Anti-SSEA4抗体[MC813-70]

• 描述

  小鼠单克隆抗体[MC813-70] to SSEA4

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat

• 免疫原

  Tissue, cells or virus corresponding to Human SSEA4. Human embryonal carcinoma cell line 2102Ep

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide

• 纯度

  Tissue culture supernatant

• 纯化说明

  Tissue culture supernatant was cross flow concentrated.

• 克隆

  单克隆

• 克隆编号

  MC813-70

• 骨髓瘤

  Sp2/0

• 同种型

  IgG3

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab16287于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 别名

• Stage specific embryonic antigen 4 antibody

• 

  Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is courtesy of Professor Chris Denning's lab, University of Nottingham

  Human induced pluripotent stem cells (iPSCs) stained with ab16287 (red fill) with secondary only control (blue fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16287, 1/9600 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG at 1/1000 dilution for 30 min at 4°C. iPSCs differentiated for 14 days toward a cardiomyocyte lineage were used as a negative control. Acquisition of >20,000 total events were collected using a 100mW solid state diode laser (488nm) and 529/28 bandpass filter.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is courtesy of an Abreview submitted by Sun Jeong Kim

  ab16287 staining SSEA4 in rat tendon derived stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 6.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.

  Immunofluorescence analysis of Human dental pulp stem cells, staining SSEA4 with ab16287 at 1/40 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany

  Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling SSEA4 with ab16287 at 1/500 dilution. Cells were fixed with paraformaldehyde and  permeabilized with 0.5% TX100. Cells were blocked with 5% serum for 20 minutes at 25°C. A goat polyclonal anti-mouse Cy3 secondary antibody at 1/500 dilution was used. 

  See Abreview

• 

  Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)

  anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)

  2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is a courtesy of Fiona Lewis

  ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor® 588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.

  See Abreview