Anti-Separase antibody [XJ11-1B12]

• 产品名称

  Anti-Separase抗体[XJ11-1B12]
  参阅全部 Separase 一抗

• 描述

  小鼠单克隆抗体[XJ11-1B12] to Separase

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details
  不适用于: Flow Cyt or ICC/IF

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: MDA-MB-231 whole cell lysate.

• 常规说明

  Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 纯度

  Affinity purified

• Primary antibody说明

  Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.

• 克隆

  单克隆

• 克隆编号

  XJ11-1B12

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab16170于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt or ICC/IF.

• 数据库链接

• Entrez Gene: 9700 Human

• Omim: 604143 Human

• SwissProt: Q14674 Human

• Unigene: 153479 Human

• 别名

• ESP1 antibody

• ESPL 1 antibody

• Espl1 antibody

• ESPL1_HUMAN antibody

• Extra spindle poles like 1 antibody

• Extra spindle poles like 1 protein antibody

• Extra spindle poles-like 1 protein antibody

• FLJ46492 antibody

• KIAA0165 antibody

• Separase antibody

• Separin antibody

• Similar to fission yeast cut1and gene antibody

• SSE antibody

• Caspase like protein ESPL1 antibody

• Caspase-like protein ESPL1 antibody

• ESP 1 antibody

• 

  Western blot - Anti-Separase antibody [XJ11-1B12] (ab16170)

  Anti-Separase antibody [XJ11-1B12] (ab16170) at 10 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 233 kDa
  Observed band size: 170 kDawhy is the actual band size different from the predicted?
  Additional bands at: 100 kDa, 70 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 3 minutes