Anti-PADI2 / PAD2抗体
参阅全部 PADI2 / PAD2 一抗
兔多克隆抗体to PADI2 / PAD2
Rabbit
ab16478 recognises a specific 75kDa band corresponding to PADI2, which is specifically blocked using the immunizing peptide in human colon, skeletal muscle and kidney lysates. There is a non-specific 18kDa band present in skeletal muscle lysates, which is attributed to cross-reactivity of the PADI2 antibody.
Replenishment batches of ab16478 are tested in WB. Previous batches were additionally validated in ELISA, Flow Cyt, ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee.
适用于: IHC-P, ELISA, ICC/IF, Flow Cyt, WBmore details
与反应: Rat, Human
Synthetic peptide corresponding to Human PADI2/ PAD2 aa 100-200 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab17091)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. PubMed: 19085382 | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| Flow Cyt | (1) | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| WB | (1) | Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa). |
Entrez Gene: 11240 Human
Omim: 607935 Human
SwissProt: Q9Y2J8 Human
Unigene: 33455 Human
Unigene: 98523 Rat
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Western blot - Anti-PADI2 / PAD2 antibody (ab16478)
Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 75 kDawhy is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab16478 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. The 75-kDa band observed is consistent with what has been described in the literature (PMID:18668562; 20668670; 16723463).
Immunocytochemistry/ Immunofluorescence - Anti-PADI2 / PAD2 antibody (ab16478)
ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
