小鼠EGFR多肽
参阅全部 EGFR 蛋白酶
ab15739 is the immunizing peptide for ab15669 and can be used to block specific EGFR protein staining by ab15669
> 90 % HPLC.
No
Synthetic
Mouse
Our Abpromise guarantee covers the use of ab15739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用
Blocking
Liquid
ab15739 is the immunizing peptide for ab15669 and can be used to block specific EGFR protein staining by ab15669
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
浓度
1 mg 浓度为 1 mg/ml
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Information available upon request.
Avian erythroblastic leukemia viral (v erb b) oncogene homolog
Cell growth inhibiting protein 40
Cell proliferation inducing protein 61
EGF R
EGFR
EGFR_HUMAN
Epidermal growth factor receptor
Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)
Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)
erb-b2 receptor tyrosine kinase 1
ERBB
ERBB1
Errp
HER1
mENA
NISBD2
Oncogen ERBB
PIG61
Proto-oncogene c-ErbB-1
Receptor tyrosine protein kinase ErbB 1
Receptor tyrosine-protein kinase ErbB-1
SA7
Species antigen 7
Urogastrone
v-erb-b Avian erythroblastic leukemia viral oncogen homolog
wa2
Wa5
Western blot - Mouse EGFR peptide (ab15739)
All lanes : Anti-EGFR antibody (ab15669) at 2 µg/ml
Lane 1 : mouse brain lysate
Lane 2 : mouse brain lysate with Mouse EGFR peptide (ab15739) at 1 µg/ml
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 0.1 µg/ml
Performed under reducing conditions.