Anti-beta Actin antibody - Cytoskeleton Marker

• 产品名称

  Anti-beta Actin抗体- Cytoskeleton Marker
  参阅全部 beta Actin 一抗

• 描述

  兔多克隆抗体to beta Actin - Cytoskeleton Marker

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, IHC-P, IPmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis

• 免疫原

  Synthetic peptide within Human beta Actin aa 1-100. The exact sequence is proprietary.
  Database link: P60709

• 阳性对照

• WB: Recombinant Human alpha smooth muscle Actin protein (ab114148), U-2 OS and SK-N-SH cell lysate. IHC-P: Human leiomyoma tissue.

• 常规说明

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.60
  Preservative: 0.1% Sodium azide
  Constituents: PBS, 1% BSA

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16039于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 396526 Chicken

• Entrez Gene: 450133 Chimpanzee

• Entrez Gene: 280979 Cow

• Entrez Gene: 403580 Dog

• Entrez Gene: 100033878 Horse

• Entrez Gene: 60 Human

• Entrez Gene: 11461 Mouse

• Entrez Gene: 414396 Pig

• Entrez Gene: 100009272 Rabbit

• Entrez Gene: 81822 Rat

• Entrez Gene: 443052 Sheep

• Entrez Gene: 398459 Xenopus laevis

• Entrez Gene: 407952 Xenopus tropicalis

• Entrez Gene: 57934 Zebrafish

• Omim: 102630 Human

• SwissProt: P60706 Chicken

• SwissProt: Q5R1X3 Chimpanzee

• SwissProt: P60712 Cow

• SwissProt: O18840 Dog

• SwissProt: P60708 Horse

• SwissProt: P60709 Human

• SwissProt: P60710 Mouse

• SwissProt: Q6QAQ1 Pig

• SwissProt: P29751 Rabbit

• SwissProt: P60711 Rat

• SwissProt: O93400 Xenopus laevis

• SwissProt: Q6NVA9 Xenopus tropicalis

• SwissProt: Q7ZVI7 Zebrafish

• Unigene: 520640 Human

• Unigene: 708120 Human

• Unigene: 727576 Human

• Unigene: 328431 Mouse

• Unigene: 391967 Mouse

• Unigene: 94978 Rat

• Unigene: 4138 Xenopus laevis

• Unigene: 35143 Zebrafish

别名

• A26C1A antibody

• A26C1B antibody

• ACTB antibody

• ACTB_HUMAN antibody

• Actin beta antibody

• Actin cytoplasmic 1 antibody

• Actin, cytoplasmic 1, N-terminally processed antibody

• Actx antibody

• b actin antibody

• b-actin antibody

• Beta cytoskeletal actin antibody

• Beta-actin antibody

• BRWS1 antibody

• E430023M04Rik antibody

• Melanoma X actin antibody

• MGC128179 antibody

• PS1TP5 binding protein 1 antibody

• PS1TP5BP1 antibody

• 

  Western blot - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)

  All lanes : Anti-beta Actin antibody - Cytoskeleton Marker (ab16039) at 1 µg/ml
  
  Lane 1 : SK-N-SH (human neuroblastoma) whole cell lysate
  Lane 2 : Human spleen tissue lysate - total protein (ab29699)
  Lane 3 : U-2 OS (human osteosarcoma cell line) whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size: 42 kDa
  Observed band size: 42 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)

  Formalin-fixed, paraffin-embedded human leiomyoma tissue stained for beta Actin with ab16039 (1/100 dilution) in immunohistochemical analysis.

• 

  Immunoprecipitation - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)

  beta Actin was immunoprecipitated using 0.5mg SK-N-SH (human neuroblastoma cell line) whole cell extract, 5 µg of Rabbit polyclonal to beta Actin and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).

  The antibody was incubated under agitation with Protein G beads for 10 minutes, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.

  Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.

  Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

  Band: 42kDa; beta Actin