Anti-Integrin alpha V抗体[272-17E6] - BSA and Azide free
参阅全部 Integrin alpha V 一抗
小鼠单克隆抗体[272-17E6] to Integrin alpha V - BSA and Azide free
Mouse
Cross reacts with all alpha V integrins.
适用于: Flow Cyt (Intra)more details
与反应: Human
不与反应: Mouse
Full length native protein (purified) corresponding to Human Integrin alpha V. Human Placental ?vß3
Database link: P06756
Flow Cyt (intra): Human melanoma cells and HeLa cells
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: PBS
是
浓度
50 µg 浓度为 1 mg/ml
Protein G purified
单克隆
272-17E6
IgG1
kappa
Abpromise™承诺保证使用ab16821于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 3685 Human
Omim: 193210 Human
SwissProt: P06756 Human
Unigene: 436873 Human
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Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)
Intracellular Flow Cytometry analysis of HeLa cells labeling Integrin alpha V with ab16821 at 0.5 µg (red), or with 1 µg of Mouse IgG isotype control.
Flow Cytometry (Intracellular) - Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)This image is a courtesy of Anonymous Abreview
ab16821 staining Integrin alpha V in Human melanoma cells by intacellular Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0. 1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.
This image was generated using the ascites version of the product.