Anti-SOCS3抗体
参阅全部 SOCS3 一抗
兔多克隆抗体to SOCS3
Rabbit
适用于: WB, ICC/IFmore details
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Dog
Synthetic peptide corresponding to Human SOCS3 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab16199)
WB: KARPAS-299 whole cell lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16030于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (9) | Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
| ICC/IF | (2) | Use a concentration of 1 µg/ml. |
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SwissProt: O14543 Human
SwissProt: O35718 Mouse
Unigene: 527973 Human
Unigene: 3468 Mouse
Unigene: 127801 Rat
Entrez Gene: 395299 Chicken
Entrez Gene: 9021 Human
Entrez Gene: 12702 Mouse
Omim: 604176 Human
SwissProt: Q90X67 Chicken
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Western blot - Anti-SOCS3 antibody (ab16030)
Lanes 1-3 : Anti-SOCS3 antibody [EPR24090-74] (ab280884) at 1/1000 dilution
Lanes 4-6 : Anti-SOCS3 antibody (ab16030) at 1/1000 dilution
Lanes 1 & 4 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysate
Lanes 2 & 5 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lanes 3 & 6 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 23,26 kDawhy is the actual band size different from the predicted?
Exposure time: 180 seconds
Blocking and dilution buffer: 5% NFDM/TBST.
ab181602 was used as loading control at 1/1000000 dilution.
Western blot - Anti-SOCS3 antibody (ab16030)
All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lane 8 : MOLT-4 (Human) Whole Cell Lysate (ab52252) with Human SOCS3 peptide (ab16199) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 30 kDawhy is the actual band size different from the predicted?
The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3.
Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody (ab16030)
ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Western blot - Anti-SOCS3 antibody (ab16030)This image is courtesy of an anonymous Abreview
All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml
Lane 1 : Untreated Human peripheral blood mononuclear cell lysate
Lane 2 : Human peripheral blood mononuclear cell lysate (treated with aCD3/28)
Lane 3 : Human peripheral blood mononuclear cell lysate (treated with IFNγ)
Lane 4 : SOCS3 protein
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG
at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 25 kDawhy is the actual band size different from the predicted?
Exposure time: 15 minutes
