Anti-COX IV antibody [20E8C12]

• 产品名称

  Anti-COX IV抗体[20E8C12]
  参阅全部 COX IV 一抗

• 描述

  小鼠单克隆抗体[20E8C12] to COX IV

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  预测可用于: Pig

• 免疫原

  Tissue, cells or virus corresponding to Cow COX IV.

• 阳性对照

• Human, bovine, murine and rat heart mitochondria.

• 常规说明

  This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituent: HEPES buffered saline

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  IgG fraction

• Primary antibody说明

  This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).

• 克隆

  单克隆

• 克隆编号

  20E8C12

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14744于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 433419 Human

• Unigene: 386758 Mouse

• Unigene: 2528 Rat

• Entrez Gene: 1327 Human

• Entrez Gene: 12857 Mouse

• Entrez Gene: 29445 Rat

• Omim: 123864 Human

• SwissProt: P00423 Cow

• SwissProt: P13073 Human

• SwissProt: P19783 Mouse

• SwissProt: P10888 Rat

• 别名

• COX IV antibody

• COX IV-1 antibody

• Cox4 antibody

• COX41_HUMAN antibody

• Cox4a antibody

• COX4B antibody

• COX4I1 antibody

• COX4I2 antibody

• COX4L2 antibody

• COXIV antibody

• Cytochrome c oxidase polypeptide IV antibody

• Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody

• Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody

• Cytochrome C Oxidase subunit IV antibody

• Cytochrome c oxidase subunit IV isoform 1 antibody

• Cytochrome c oxidase subunit IV isoform 2 (lung) antibody

• Cytochrome c oxydase subunit 4 antibody

• dJ857M17.2 antibody

• MGC105470 antibody

• MGC72016 antibody

• AL024441 antibody

• COX 4 antibody

• COX IV 1 antibody

• 

  Western blot - Anti-COX IV antibody [20E8C12] (ab14744)

  All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1/5000 dilution
  
  Lane 1 : Cytoplasmic fraction mouse NIH/3T3 cell lysate
  Lane 2 : Nuclear fraction mouse NIH/3T3 cell lysate
  
  Secondary
  All lanes : HRP conjugated goat anti-mouse antibody
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 16 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 3 minutes
  
  
  This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005.

  See Abreview

• 

  Flow Cytometry - Anti-COX IV antibody [20E8C12] (ab14744)

  Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Western blot - Anti-COX IV antibody [20E8C12] (ab14744)

  All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1 µg/ml
  
  Lane 1 : Isolated mitochondria from human heart at 5 µg
  Lane 2 : Isolated mitochondria from bovine heart at 1 µg
  Lane 3 : Isolated mitochondria from rat heart at 10 µg
  Lane 4 : Isolated mitochondria from murine heart at 10 µg
  
  Observed band size: 16 kDawhy is the actual band size different from the predicted?