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Jurkat nuclear extract lysate (TPA + CI stimulated)

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200ug
- +
产品详情
  • 产品名称

    Jurkat核extract裂解物(TPA + CI stimulated)
    参阅全部 Jurkat 细胞裂解液

  • 常规说明

    Cell line: Jurkat (Human T cell lymphoblast-like).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
    Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.

  • Mycoplasma free

    Yes

  • 形式

    Liquid

  • 存放说明

    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol

  • 浓度

    • 200 µg 浓度为 6.09 mg/ml


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