Anti-UQCRC2 antibody [13G12AF12BB11]

• 产品名称

  Anti-UQCRC2抗体[13G12AF12BB11]
  参阅全部 UQCRC2 一抗

• 描述

  小鼠单克隆抗体[13G12AF12BB11] to UQCRC2

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-P, WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Pig

• 免疫原

  Tissue, cells or virus corresponding to Cow UQCRC2.

• 阳性对照

• Human, bovine, murine and rat heart mitochondria. HepG2 cell lysate.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• 存储溶液

  pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituent: HEPES buffered saline

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  IgG fraction

• 克隆

  单克隆

• 克隆编号

  13G12AF12BB11

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14745于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7385 Human

• Omim: 191329 Human

• SwissProt: P22695 Human

• Unigene: 528803 Human

• 别名

• Cytochrome b-c1 complex subunit 2 antibody

• EC 1.10.2.2 antibody

• MC3DN5 antibody

• mitochondrial antibody

• QCR2 antibody

• QCR2_HUMAN antibody

• Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody

• Ubiquinol cytochrome c reductase core protein II antibody

• Ubiquinol-cytochrome-c reductase complex core protein 2 antibody

• UQCR2 antibody

• Uqcrc2 antibody

• Complex III subunit 2 antibody

• Core protein II antibody

• Cytochrome b c1 complex subunit 2 mitochondrial antibody

• 

  Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)This image is courtesy of an anonymous Abreview

  Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 1/1000 dilution + H23 whole cell lysate at 20 µg
  
  Secondary
  HRP-conjugated goat anti-mouse IgG polyclonal at 1/2000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 48 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 1 minute
  

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

  IHC image of ab14745 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14745, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Flow Cytometry - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

  Overlay histogram showing HepG2 cells stained with ab14745 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14745, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunofluorescence - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

  Mitochondrial localization of complex III visualized by immunofluorescence using anti-complex III subunit Core 2 mAb 2E3GC12FB2AE2 (ab14745). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14745 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

• 

  Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

  All lanes : Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 0.5 µg/ml
  
  Lane 1 : Human skeletal muscle tissue lysate
  Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to mouse IgG - H&L - pre-adsorbed (HRP) at 1/3000 dilution
  
  Observed band size: 48 kDawhy is the actual band size different from the predicted?
  Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.