Anti-UQCRFS1/RISP antibody [5A5]

• 产品名称

  Anti-UQCRFS1/RISP抗体[5A5]
  参阅全部 UQCRFS1/RISP 一抗

• 描述

  小鼠单克隆抗体[5A5] to UQCRFS1/RISP

• 宿主

  Mouse

• 经测试应用

  适用于: WB, Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  预测可用于: Pig

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Human, bovine, rat and murine heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in Raji cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  
  

  Product was previously marketed under the MitoSciences sub-brand.

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 7.5
  Preservative: 0.02% Sodium azide
  Constituent: HEPES buffered saline

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

• 克隆

  单克隆

• 克隆编号

  5A5

• 同种型

  IgG2b

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14746于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 726119 Human

• Unigene: 181933 Mouse

• Unigene: 2603 Rat

• Entrez Gene: 7386 Human

• Entrez Gene: 66694 Mouse

• Entrez Gene: 291103 Rat

• Omim: 191327 Human

• SwissProt: P13272 Cow

• SwissProt: P47985 Human

• SwissProt: Q9CR68 Mouse

• SwissProt: P20788 Rat

• 别名

• Cytochrome b-c1 complex subunit 11 antibody

• Cytochrome b-c1 complex subunit 5 antibody

• Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody

• petC antibody

• PGR1 antibody

• Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody

• Proton gradient regulation protein 1 antibody

• Rieske iron sulfur protein antibody

• Rieske iron-sulfur protein antibody

• RIP1 antibody

• RIS1 antibody

• RISP antibody

• Ubiquinol cytochrome C reductase rieske iron sulphur antibody

• Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody

• Ubiquinol-cytochrome c reductase 8 kDa protein antibody

• Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody

• UCRI_HUMAN antibody

• UQCR5 antibody

• UQCRFS1 antibody

• Complex III subunit 5 antibody

• Complex III subunit IX antibody

• Cytochrome b c1 complex subunit Rieske mitochondrial antibody

• 

  Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)

  All lanes : Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 0.5 µg/ml
  
  Lane 1 : Hela Whole Cell Lysate - Hydroxyurea Treated
  Lane 2 : Hela Whole Cell Lysate - Bleomycin Treated
  Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Observed band size: 25 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)

  Human normal kidney (cortex). Staining is observed intracellular. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Western blot - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)

  All lanes : Anti-UQCRFS1/RISP antibody [5A5] (ab14746) at 1 µg/ml
  
  Lane 1 : Isolated mitochondria from human heart at 5 µg
  Lane 2 : Isolated mitochondria from bovine heart at 1 µg
  Lane 3 : Isolated mitochondria from rat heart at 10 µg
  Lane 4 : Isolated mitochondria from mouse heart at 10 µg
  

• 

  Flow Cytometry - Anti-UQCRFS1/RISP antibody [5A5] (ab14746)

  Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.