Anti-LAMP1抗体[LY1C6]
参阅全部 LAMP1 一抗
小鼠单克隆抗体[LY1C6] to LAMP1
Mouse
适用于: ICC, WBmore details
与反应: Rat, Human
Tissue, cells or virus corresponding to Rat LAMP1. Rat liver lysosomal membrane preparations
Highly purified rat lysosomal membrane preparations.
For maximum product recovery centrifuge the product vial before removing cap.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Protein G purified
单克隆
LY1C6
IgG1
Abpromise™承诺保证使用ab13523于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC | (1) | Use at an assay dependent concentration. |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa. |
Entrez Gene: 3916 Human
Omim: 153330 Human
SwissProt: P11279 Human
Unigene: 494419 Human
Unigene: 40177 Rat
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LGP120 antibody
lgpA antibody
Lysosomal membrane glycoprotein 120KD antibody
Lysosomal Associated Membrane Protein 1 antibody
Lysosome associated membrane glycoprotein 1 antibody
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OTTHUMP00000040663 antibody
CD107 antigen like family member A antibody
CD107 antigen-like family member A antibody
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Western blot - Anti-LAMP1 antibody [LY1C6] (ab13523)
Anti-LAMP1 antibody [LY1C6] (ab13523) at 1/1000 dilution + Rat liver microsome lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Immunocytochemistry - Anti-LAMP1 antibody [LY1C6] (ab13523)
ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.
Western blot - Anti-LAMP1 antibody [LY1C6] (ab13523)Image from Banno A et al, J Biol Chem. 2012 Apr 20;287(17):13799-812. Epub 2012 Feb 18, Fig 4. DOI 10.1074/jbc.M112.341214 April 20, 2012 The Journal of Biological Chemistry, 287, 13799-13812.
ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.
WCL, N/IC, C, P, and PM indicate whole cell lysates, nuclear/intact cell, cytosolic, high-speed pellet, and plasma membrane fractions, respectively.