Anti-TLR4 antibody

• 产品名称

  Anti-TLR4抗体
  参阅全部 TLR4 一抗

• 描述

  兔多克隆抗体to TLR4

• 宿主

  Rabbit

• 特异性

  TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody.

• 经测试应用

  适用于: WB, Flow Cytmore details
  不适用于: IHC-Fr or IHC-P

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to Human TLR4 aa 420-435.
  Sequence:

  GLEQLEHLDFQ HSNLK

  
  Database link: O00206

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HEK-293T overexpressing TLR4 (unboiled) cell lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab13556于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IHC-Fr or IHC-P.

• 数据库链接

• Entrez Gene: 7099 Human

• Omim: 603030 Human

• SwissProt: O00206 Human

• Unigene: 174312 Human

• 别名

• Homolog of Drosophila toll antibody

• hToll antibody

• TLR 4 antibody

• TLR4 antibody

• TLR4_HUMAN antibody

• TOLL antibody

• Toll like receptor 4 antibody

• Toll-like receptor 4 antibody

• ARMD10 antibody

• CD284 antibody

• CD284 antigen antibody

• 

  Western blot - Anti-TLR4 antibody (ab13556)

  All lanes : Anti-TLR4 antibody (ab13556) at 1/1000 dilution
  
  Lane 1 : HEK-293T overexpressing TLR4 (unboiled) cell lysate
  Lane 2 : HEK-293T untransfected (unboiled) cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 75-80 kDa
  Observed band size: 70 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Western blot: Anti-TLR4 antibody (ab13556) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab13556 was shown to bind specifically to TLR4. First, unboiled samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

• 

  Flow Cytometry - Anti-TLR4 antibody (ab13556)

  Flow cytometry analysis of THP-1 (Human monocytic leukemia cell line). Cells were fixed with 2% formaldehyde for 10 minutes at room temperature. ab13556 was used at  2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1/200 dilution for 40 minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).