Anti-GRP94 antibody

• 产品名称

  Anti-GRP94抗体
  参阅全部 GRP94 一抗

• 描述

  兔多克隆抗体to GRP94

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide corresponding to Mouse GRP94 aa 787-802 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
  Sequence:

  EEEEETEKESTEKDEL

  
  Database link: P08113

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab13509于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 87773 Mouse

• Unigene: 101146 Rat

• Entrez Gene: 7184 Human

• Entrez Gene: 22027 Mouse

• Entrez Gene: 362862 Rat

• Omim: 191175 Human

• SwissProt: P14625 Human

• SwissProt: P08113 Mouse

• SwissProt: Q66HD0 Rat

• Unigene: 192374 Human

• 别名

• Endoplasmin antibody

• Endothelial cell (HBMEC) glycoprotein antibody

• ENPL_HUMAN antibody

• Glucose regulated protein 94kDa antibody

• gp96 antibody

• gp96 homolog antibody

• GRP 94 antibody

• GRP-94 antibody

• Heat shock protein 90 kDa beta member 1 antibody

• heat shock protein 90kDa beta (Grp94), member 1 antibody

• Heat shock protein, 90 kDa, beta, 1 antibody

• HSP90B1 antibody

• Stress inducible tumor rejection antigen GP96 antibody

• TRA1 antibody

• tumor rejection antigen (gp96) 1 antibody

• Tumor rejection antigen 1 antibody

• Tumor rejection antigen gp96 antibody

• Tumor rejection antigen-1 (gp96) antibody

• 94 kDa glucose regulated protein antibody

• 94 kDa glucose-regulated protein antibody

• ECGP antibody

• 

  Western blot - Anti-GRP94 antibody (ab13509)

  All lanes : Anti-GRP94 antibody (ab13509) at 1/1000 dilution
  
  Lane 1 : Molecular weight ladder
  Lane 2 : Lysates prepared from rat brain
  Lane 3 : Lysates prepared from mouse brain
  Lane 4 : Lysates prepared from rat liver microsomes
  Lane 5 : Lysates prepared from mouse liver microsomes
  Lane 6 : Lysates prepared from human liver microsomes
  
  Predicted band size: 94 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab13509)

  ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

• 

  Western blot - Anti-GRP94 antibody (ab13509)