Anti-Cytochrome P450 Reductase抗体
参阅全部 Cytochrome P450 Reductase 一抗
兔多克隆抗体to Cytochrome P450 Reductase
Rabbit
适用于: WB, ICC/IFmore details
与反应: Rat, Human
Full length native protein (purified) corresponding to Rat Cytochrome P450 Reductase.
Database link: P00388
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
浓度
50 µl 浓度为 1 mg/ml
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab13513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/1000. Detects a band of approximately 78 kDa. |
| ICC/IF | Use a concentration of 10 µg/ml. |
Entrez Gene: 5447 Human
Omim: 124015 Human
SwissProt: P16435 Human
Unigene: 354056 Human
Unigene: 11359 Rat
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Western blot - Anti-Cytochrome P450 Reductase antibody (ab13513)
Anti-Cytochrome P450 Reductase antibody (ab13513) at 1/2000 dilution + Cell lysates prepared from rat PC12 cells
Western blot analysis of rat PC12 cell lysate, probed with ab13513.
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody (ab13513)
ICC/IF image of ab13513 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13513, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
