Anti-ATR抗体
参阅全部 ATR 一抗
兔多克隆抗体to ATR
Rabbit
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
适用于: WBmore details
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human ATR aa 400-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13535
Whole cell lysate from HeLa and U2OS cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab10312于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/15000 - 1/30000. Detects a band of approximately 300 kDa (predicted molecular weight: 317 kDa). |
Entrez Gene: 545 Human
Omim: 601215 Human
SwissProt: Q13535 Human
Unigene: 271791 Human
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Western blot - Anti-ATR antibody (ab10312)
All lanes : Anti-ATR antibody (ab10312) at 1/15000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : ATR knockout A549 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 317 kDa
Observed band size: 260 kDawhy is the actual band size different from the predicted?
False colour image of Western blot: Anti-ATR antibody staining at 1/15000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab10312 was shown to bind specifically to ATR. A band was observed at 260 kDa in wild-type A549 cell lysates with no signal observed at this size in ATR knockout cell line ab276104 (knockout cell lysate ab277987). To generate this image, wild-type and ATR knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-ATR antibody (ab10312)
Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure. Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure.
