Anti-HPRT抗体
参阅全部 HPRT 一抗
兔多克隆抗体to HPRT
Rabbit
Replenishment batches of our polyclonal antibody, ab10479 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab133242.
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Human
预测可用于: Chicken, Gerbil, Chinese hamster
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: MEF1, NIH/3T3, HeLa, MCF-7, HEK-293, A-431, Wild-type HAP1 and PC12 whole cell lysates, mouse brain tissue lysate and Recombinant Human HPRT protein (ab117153). ICC/IF: HepG2 cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.6 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab10479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. |
| ICC/IF | Use a concentration of 5 µg/ml. |
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Unigene: 412707 Human
Unigene: 299381 Mouse
Unigene: 47 Rat
Entrez Gene: 395653 Chicken
Entrez Gene: 3251 Human
Entrez Gene: 15452 Mouse
Omim: 308000 Human
SwissProt: Q9W719 Chicken
SwissProt: P00492 Human
SwissProt: P00493 Mouse
HGPRT antibody
HGPRTase antibody
HPRT 1 antibody
HPRT_HUMAN antibody
HPRT1 antibody
Hypoxanthine guanine phosphoribosyltransferase antibody
Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody
Hypoxanthine phosphoribosyltransferase 1 antibody
Hypoxanthine-guanine phosphoribosyltransferase antibody
Western blot - Anti-HPRT antibody (ab10479)
Lane 1: HeLa whole cell lysate (20 µg)
Lane 2: NIH/3T3 whole cell lysate (20 µg)
Lane 3: PC12 whole cell lysate (20 µg)
Lane 4: Wild-type HAP1 whole cell lysate (20 µg)
Lane 5: Empty Lane
Lane 6: HPRT1 knockout HAP1 whole cell lysate (20 µg)
Lanes 1 - 6: Merged signal (red and green). Green - ab10479 observed at 24 kDa. Red - loading control ab6301 observed at 95 kDa.
Gel type: MES
Blocking buffer: LiCOR blocking buffer
ab10479 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were examined. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. Ab10479 and Ab6301 (Beta catenin - Loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800RD) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680CW) preadsorbed secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-HPRT antibody (ab10479)
ab10479 staining HPRT in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab10479 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-HPRT antibody (ab10479)
All lanes : Anti-HPRT antibody (ab10479) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A-431 whole cell lysate (ab7909)
Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HEK-293 whole cell lysate (ab7902)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Western blot - Anti-HPRT antibody (ab10479)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10479 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab10479 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were examined. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. Ab10479 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-HPRT antibody (ab10479)
All lanes : Anti-HPRT antibody (ab10479) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 28 kDa (possible glycosylated form)
Western blot - Anti-HPRT antibody (ab10479)
