Anti-CBX1 / HP1 beta antibody [MAC353]

• 产品名称

  Anti-CBX1 / HP1 beta抗体[MAC353]
  参阅全部 CBX1 / HP1 beta 一抗

• 描述

  大鼠单克隆抗体[MAC353] to CBX1 / HP1 beta

• 宿主

  Rat

• 经测试应用

  适用于: WB, ICC/IF, IP, Indirect ELISA, IHC-Pmore details
  不适用于: IHC-Fr

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Fusion protein corresponding to Mouse CBX1/ HP1 beta (C terminal).
  Database link: P83917

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 20th June 2025

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 1 ml 浓度为 0.903 mg/ml

• 纯度

  Ion Exchange Chromatography

• 克隆

  单克隆

• 克隆编号

  MAC353

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab10811于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IHC-Fr.

• 数据库链接

• Entrez Gene: 10951 Human

• Entrez Gene: 12412 Mouse

• Entrez Gene: 360609 Rat

• Omim: 604511 Human

• SwissProt: P83916 Human

• SwissProt: P83917 Mouse

• Unigene: 77254 Human

• Unigene: 29055 Mouse

• 别名

• CBX1_HUMAN antibody

• Chromobox 1 antibody

• Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody

• chromobox homolog 1 antibody

• Chromobox protein homolog 1 antibody

• Drosophila HP1 beta antibody

• Heterochromatin protein 1 beta antibody

• Heterochromatin protein 1 homolog beta antibody

• Heterochromatin protein p25 antibody

• heterochromatin protein p25 beta antibody

• HP1 beta antibody

• HP1 beta homolog antibody

• HP1 beta homolog Drosophila antibody

• HP1, Drosophila. homolog of, beta antibody

• HP1beta antibody

• HP1Hs beta antibody

• HP1Hsbeta antibody

• Human heterochromatin protein p25 mRNA complete cds antibody

• M31 antibody

• MOD 1 antibody

• MOD1 antibody

• Modifier 1 protein antibody

• p25beta antibody

• CBX 1 antibody

• CBX antibody

• Cbx1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811)This image is courtesy of Scott Slattery and Mike Mancini

  HeLA cells were stained with ab10811 in panel one. In panel two they were stained with ab10811 (green), DAPI (blue) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeablize 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4oC diluted 1/100 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen. Notes: Ample washing between each step. TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2.

  Please note this image was from a different batch produced using hybridoma method

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] (ab10811)Image from Talasz H et al., J Biol Chem. 2005 Nov 18;280(46):38814-22. Epub 2005 Sep 15. Fig 1.; doi: 10.1074/jbc.M505563200; November 18, 2005 The Journal of Biological Chemistry, 280, 38814-38822.

  Immunofluorescence analysis of mouse erythroleukemia cell nuclei, staining CBX1 / HP1 beta in heterochromatin, with ab10811.
  
  Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and blocked for 30 min with 2.5% BSA. Cells were incubated with primary antibody (1/50) before incubating with a Cy3-conjugated goat anti-rat IgG to detect staining.

  Please note this image was from a different batch produced using hybridoma method