Anti-ICAM3抗体[ICAM3.1]
参阅全部 ICAM3 一抗
小鼠单克隆抗体[ICAM3.1] to ICAM3
Mouse
适用于: IHC-Pmore details
与反应: Human
Recombinant fragment (Fc-tag) corresponding to ICAM3.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
浓度
50 µg 浓度为 0.7 mg/ml
Protein A purified
单克隆
ICAM3.1
Sp2
IgG1
Abpromise™承诺保证使用ab10804于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
应用说明
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 66 kDa.
ICC/IF: Use at an assay dependent dilution From (PubMed:17353362)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 3385 Human
Omim: 146631 Human
SwissProt: P32942 Human
Unigene: 654563 Human
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (ab10804)](https://www.abcam.cn/ps/products/10/ab10804/Images/ab10804-18465-anti-icam3-antibody-icam31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (ab10804)
Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
