B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody B cell activation protein antibody B-cell activation protein antibody BL11 antibody BL11 PEN antibody CD83 antibody CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) antibody CD83 antigen antibody CD83 molecule antibody CD83_HUMAN antibody Cell surface glycoprotein antibody Cell surface protein HB15 antibody HB15 antibody hCD83 antibody
Product description
The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.
Immunogen
Purified recombinant fragment of human CD83 (AA: extra 20-144) expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WB
WB:1:500-1:2,000
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody B cell activation protein antibody B-cell activation protein antibody BL11 antibody BL11 PEN antibody CD83 antibody CD83 antigen (activated B lymphocytes immunoglobulin superfamily) antibody CD83 antigen antibody CD83 molecule antibody CD83_HUMAN antibody Cell surface glycoprotein antibody Cell surface protein HB15 antibody HB15 antibody hCD83 antibody
Western blot analysis of CD83 against human CD83 (AA: extra 20-144) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1710-91 against HEK293 (1) and CD83 (AA: extra 20-144)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Positive Control
Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.
Application Notes
WB:1:500-1:2,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
