| 存储条件 |
|---|
| Product Name | Angiotensinogen [SD201-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody AGT antibody AI265500 antibody Alpha 1 antiproteinase antitrypsin antibody Ang antibody Ang I antibody Ang II antibody Ang III antibody AngII antibody Angiotensin I antibody Angiotensin II antibody Angiotensin III antibody Angiotensin-3 antibody Angiotensinogen (PAT) antibody Angiotensinogen antibody ANGT_HUMAN antibody ANHU antibody ANRT antibody AT-2 antibody AT-II antibody Des-Asp[1]-angiotensin II antibody FLJ92595 antibody FLJ97926 antibody MGC105326 antibody PAT antibody Pre angiotensinogen antibody Serine (or cysteine) proteinase inhibitor antibody Serpin A8 antibody Serpin peptidase inhibitor clade A member 8 antibody SERPINA8 antibody |
| Product description | Angiotensin is formed from a precursor, angiotensinogen, which is produced by the liver and found in the a-globulin fraction of plasma. The lowering of blood pressure is a stimulus to secretion of Renin by the kidney into the blood. Renin cleaves from angiotensinogen a terminal decapeptide, Angiotensin I (Ang I). This is further altered by the enzymatic removal of a dipeptide to form Angiotensin II (Ang II). Screening a panel of human-mouse somatic cell hybrids confirmed the assignment of the AGT locus to human chromosome 1. Angiotensin, an octapeptide hormone, is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. It has been shown that mechanical stress causes release of Angiotensin from cardiac myocytes and that Angiotensin acts as an initial mediator of the hypertrophic response. Angiotensin treatment also stimulates phosphorylation of Shc, FAK and MAP kinases and induces MKP-1, indicating stimulation of growth factor pathways. Angiotensin stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by co-immunoprecipitation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIP |
| WB:1:1,000 ICC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody AGT antibody AI265500 antibody Alpha 1 antiproteinase antitrypsin antibody Ang antibody Ang I antibody Ang II antibody Ang III antibody AngII antibody Angiotensin I antibody Angiotensin II antibody Angiotensin III antibody Angiotensin-3 antibody Angiotensinogen (PAT) antibody Angiotensinogen antibody ANGT_HUMAN antibody ANHU antibody ANRT antibody AT-2 antibody AT-II antibody Des-Asp[1]-angiotensin II antibody FLJ92595 antibody FLJ97926 antibody MGC105326 antibody PAT antibody Pre angiotensinogen antibody Serine (or cysteine) proteinase inhibitor antibody Serpin A8 antibody Serpin peptidase inhibitor clade A member 8 antibody SERPINA8 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P01019 |
|---|
Application
Fig1: ICC staining Angiotensinogen in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Angiotensinogen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Angiotensinogen in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Angiotensinogen in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | LO2, 293, Hela, HepG2. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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