B7 H5 antibody B7H5 antibody C10orf54 antibody chromosome 10 open reading frame 54 antibody DD1alpha antibody GI24 antibody GI24_HUMAN antibody PD-1H antibody PDCD1 homolog antibody Platelet receptor Gi24 antibody PP2135 antibody sisp 1 antibody SISP1 antibody stress induced secreted protein 1 antibody UNQ730/PRO1412 antibody V domain Ig suppressor of T cell activation antibody V set domain containing immunoregulatory receptor antibody V set immunoregulatory receptor antibody V type immunoglobulin domain containing suppressor of T cell activation antibody VISTA antibody
Product description
VSIR (V-Set Immunoregulatory Receptor) is a Protein Coding gene. Diseases associated with VSIR include Parasagittal Meningioma and Solar Retinopathy. Among its related pathways are T Cell Co-Signaling Pathway: Ligand-Receptor Interactions and NF-kappaB Signaling.
Immunogen
Purified recombinant fragment of human VISTA (AA: extra 33-194) expressed in HEK293 cells.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBFC
WB:1:500-1:2,000 FC:1:100-1:200
Species Reactivity
Human
Concentration
1mg/mL
Target Information
Alternative Names
B7 H5 antibody B7H5 antibody C10orf54 antibody chromosome 10 open reading frame 54 antibody DD1alpha antibody GI24 antibody GI24_HUMAN antibody PD-1H antibody PDCD1 homolog antibody Platelet receptor Gi24 antibody PP2135 antibody sisp 1 antibody SISP1 antibody stress induced secreted protein 1 antibody UNQ730/PRO1412 antibody V domain Ig suppressor of T cell activation antibody V set domain containing immunoregulatory receptor antibody V set immunoregulatory receptor antibody V type immunoglobulin domain containing suppressor of T cell activation antibody VISTA antibody
Western blot analysis of VISTA against human VISTA (AA: extra 33-194) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of VISTA was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-63, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Positive Control
Jurkat cells
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
